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Abstract
Background: Helicobacter pylori plays an important role in the pathogenesis of gastric cancer. H. pylori has the iceA gene caused by epithelial contact during bacterial attachment to the gastric mucosa, with two variants of iceA1 and iceA2). Currently there are several studies that show the link between H. pylori iceA1 and peptic ulcer disease, but this hypothesis remains controversial. Methods: The objective of this study was to investigate the expression of H. pylori with iceA gene had two main allelic variants iceA1 and iceA2 in patients who were diagnosed with gastric cancer in endoscopy and to assess whether there was correlation between genotypes.
Results: A total of 183 tissue samples from patients with gastric cancer (n = 91) with H. pylori infection were confirmed by histopathological examination of the biopsy specimens included in the study. The positive of H. pylori in the samples was also confirmed by amplification of the iceA gene by polymerase chain reaction (PCR). The H. pylori allei iceA1 and iceA2 bacteria were genetically engineered using specific primers in a gastric biopsy sample using PCR. The positive rate of iceA1 and iceA2 in patients with gastric cancer was 41,6% (15/36), 22% (8/36), and iceA2. The difference in the chromosome rate of iceA1 and iceA2 among the patients was not statistically significant (p> 0.05). As a result, the H. pylori iceA1 genotype predominates (65%) in gastric cancer similar to our regional studies, but the prevalence of the iceA2 genotype is lower (28%). Our results support the idea that the iceA gene reflects geographic differentiation rather than clinical and virulence.
Conclusion: a larger sample size should be included with gastric and gastric ulcer groups in order to better evaluate H. pylori iceA gene expression and the association of gastric cancer.
References
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| Published | 07-01-2025 | |
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| Issue | No. 67 (2021) | |
| Section | Original article | |
| DOI | 10.38103/jcmhch.2021.67.7 | |
| Keywords | Gene iceA, iceA1, iceA2, Helicobacter pylori, Ung thư dạ dày gene iceA, iceA1, iceA2, Helicobacter pylori, gastric cancer |
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