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Abstract
Since Charpentier and Doudna discovered the CRISPR/Cas9 genetic scissors in 2012 their use has exploded. Researchers can change the DNA of animals, plants and microorganisms with extremely high precision. In medicine, clinical trials of new cancer therapies are underway, and the dream of being able to cure inherited diseases is about to come true. These genetic scissors have taken the life sciences into a new epoch and, in many ways, are bringing the greatest benefit to humankind.
In 2015, Doudna called for a worldwide moratorium on the use CRISPR/Cas9, a technology she developed to rewrite genetic code in human embryos. She discuss the enormous responsibility that comes with the ability to rewrite the code of life.
References
Nguyễn Chấn Hùng. Con người viết lại mã sự sống. Sâu Thẳm Sự Sống. NXB Tổng Hợp TP. Hồ Chí Minh. 2022.
Cobb M. 1953: when genes became “information”. Cell. 2013;153:503-6.
Gao YZ. [The double helix model of DNA structure - the physical nature of the gene]. Yi Chuan. 2002;24:691-4.
Tamura K. The Genetic Code: Francis Crick’s Legacy and Beyond. Life (Basel). 2016;6.
Charpentier E. CRISPR - Cas9: how research on a bacterial RNA - guided mechanism opened new perspectives in biotechnology and biomedicine. EMBO Mol Med. 2015;7:363-5.
Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR - Cas9. Science. 2014;346:1258096.
Cobb M. 60 years ago, Francis Crick changed the logic of biology. PLoS Biol. 2017;15:e2003243.
Crick F. Central dogma of molecular biology. Nature. 1970;227:561-3.
Kornberg R. The molecular basis of eukaryotic transcription (Nobel Lecture). Angew Chem Int Ed Engl. 2007;46:6956-65.
Rodnina MV, Wintermeyer W. The ribosome goes Nobel. Trends Biochem Sci. 2010;35:1-5.
Steitz TA, Moore PB. Perspectives on the ribosome. Philos Trans R Soc Lond B Biol Sci. 2017;372.
Lehmann AR. DNA repair, DNA replication and human disorders: a personal journey. DNA Repair (Amst). 2012;11:328-34.
Lindahl T. My journey to DNA repair. Genomics Proteomics Bioinformatics. 2013;11:2-7.
Saini N. The journey of DNA repair. Trends Cancer. 2015;1:215-216.
Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual - RNA - guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337:816-21.
Westermann L, Neubauer B, Kottgen M. Nobel Prize 2020 in Chemistry honors CRISPR: a tool for rewriting the code of life. Pflugers Arch. 2021;473:1-2.
Le Rhun A, Escalera - Maurer A, Bratovic M, Charpentier
E. CRISPR - Cas in Streptococcus pyogenes. RNA Biol. 2019;16:380-389.
Jiang F, Doudna JA. CRISPR - Cas9 Structures and Mechanisms. Annu Rev Biophys. 2017;46:505-529.
Urena - Bailen G, Lamsfus - Calle A, Daniel - Moreno A, Raju J, Schlegel P, Seitz C, et al. CRISPR/Cas9 technology: towards a new generation of improved CAR - T cells for anticancer therapies. Brief Funct Genomics. 2020;19:191-200.
Liu Z, Liao Z, Chen Y, Zhou L, Huangting W, Xiao H. Research on CRISPR/system in major cancers and its potential in cancer treatments. Clin Transl Oncol. 2021;23:425-433.
Karimian A, Gorjizadeh N, Alemi F, Asemi Z, Azizian K, Soleimanpour J, et al. CRISPR/Cas9 novel therapeutic road for the treatment of neurodegenerative diseases. Life Sci. 2020;259:118165.
Herrera - Carrillo E, Gao Z, Berkhout B. CRISPR therapy towards an HIV cure. Brief Funct Genomics. 2020;19:201-208.
Dong MB, Wang G, Chow RD, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome - Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204 e23.
Cheng X, Fan S, Wen C, Du X. CRISPR/Cas9 for cancer treatment: technology, clinical applications and challenges. Brief Funct Genomics. 2020;19:209-214.
Chen M, Xu J, Zhou Y, Zhang S, Zhu D. CRISPR
- Cas9 genome editing for cancer immunotherapy: opportunities and challenges. Brief Funct Genomics. 2020;19:183-190.
Behan FM, Iorio F, Picco G, Goncalves E, Beaver CM, Migliardi G, et al. Prioritization of cancer therapeutic targets using CRISPR - Cas9 screens. Nature. 2019;568:511-516.
Ye ZJ, Zhang XY, Liang J, Tang Y. The Challenges of Medical Ethics in China: Are Gene - Edited Babies Enough? Sci Eng Ethics. 2020;26:123-125.
Wu SS, Li QC, Yin CQ, Xue W, Song CQ. Advances in CRISPR/Cas - based Gene Therapy in Human Genetic Diseases. Theranostics. 2020;10:4374-4382.
| Published | 11-08-2022 | |
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| Issue | No. 82 (2022) | |
| Section | Review | |
| DOI | 10.38103/jcmhch.82.1 | |
| Keywords | Kéo di truyền, CRISPR/Cas9, sự sống Genetic scissors, CRISPR/Cas9, life |
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