Abstract
Isolation and culture mononuclear cells from the bone marrow of five patients without diseases of the bone marrow and hematological, average the mononuclear cell are obtained. (2.88±0.61)×105 cells/mL and the average the number of mesenchymal stem cells from five patients bone marrow: (4.8±0.07)×104 cells/mL in vitro culture after 22 days. These MSC cells could differentiato into adiposo. Average number of CFU-f per mL: 45 CFU. Then we applied this protocol of MSC culture and proliferation process for patient chronic renal failure: average the mononuclear cell are obtained: (2.26±0.15)×105cells/mL and the average the number of mesenchymal stem cells from five patients bone marrow: (4.5±0.51)×105/cells m in vitro culture after 22 days. The number of CFU-f per mL: 42 CFU.
References
Bi B, Schmitt R, Israilova M et al. Stromal cells protect against acute tubular injury via an endocrine effect. J. Am. Soc. Nephrol 2007; 18: p.2486-96.
English K, Barry FP, Field-Corbett CPetal (2001), IFN-gamma and TNF-alpha differentially regulate immunomodulation by murine mesenchymal stem cells, Immunol Lett, 110, pp. 91-100
Herrera MB, Bussolati B, Bruno S, Morando L, Mauriello-Romanazzi G, Sanavio F, Stamenkovic I, Biancone L, Camussi G (2007), Exogenous mesenchymal stem cells localize to the kidney by means of CD44 following acute tubular injury, Kidney Int, 72, pp.430 441.
Jose J. Minguell, Alejandro E, Paulette Conget (2002), Mesenchymal Stem Cells, Santiago, Chile.
Kuci S, Kuci Z, Latifi-Pupovci H et al. (2009), Adult stem cells as an alternative source of multipotential (pluripotential) cells in regenerative medicine, Curr. Stem Cell Res. Ther,4, pp.107-17.
Meirelles L.d.S. and Nardi N.B. (2003), Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization, British journal of haematology, 123(4), pp.702-711.
Mervat EL, Gamla Saadi, at al (2012), Mesenchymal stem cells are a rescue approach for recovery of deteriorating kidney function, Nephrology, 17 2012, pp.650-657.
Mohammadi-Sangcheshmeh A., et al. (2013), Isolation, characterization, and mesodermic differentiation of stem cells from adipose tissue of camel (Camelus dromedarius), In Vitro Cellular & Developmental Biology-Animal, 49(2), pp. 147-154.
Perico, N.etal. (2016), Autologous mesenchymal stromal cells and kidney transplantation: a pilot study of safety and clinical feasibility, Clin. J Am Soc Nephrol, 6, pp.412-22.
Scott R, Ben Nguyen (2015), Pulsed Focused Ultrasound Pretreatment Improves Mesenchymal Stromal Cell Efficacy in Preventing and Rescuing Established Acute Kidney Injury in Mice, Stem cell, 33, pp.1241-1253.
Steven M. Devinea, Amelia M. Bartholomewb, Nadim Mahmuda, Mary Nelsona, Sheila Patilf, Wayne Hardyf, Cord Sturgeonb, Terry Hewette, Theodore Chungc, Wendy Stocka, Dorie Shera, Scott Weissmana, Karen Ferrerd, Joseph Moscaf, Robert Deansf, Annemarie Moseleyf and Ronald Hoffmana (2001), Mesenchymal stem cells are capable of homing to the bone marrow of non-human primates following systemic infusion, Experimental Hematology, 29 pp.244-255.
Wen Li, Li Wang, at al (2016), Icariin combined with human umbilical cord mesenchymal stem cells significantly, Mol Cell Biochem, 19, pp.443-56.
Yang X.-F., et al. (2011), High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells, Journal of biomedical1 science, 18(1), pp.59
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright (c) 2025 Journal of Clinical Medicine Hue Central Hospital