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Abstract
Objectives: Evaluation of the cancer treatment effect of measles and mumps virus vaccine (MeV and
MuV) combination against human colorectal cancer (HT-29 Cell) in nude micexenograft models.
Materials and methods: MeV and MuV were proliferated and titrated by TCID50 assay. 49 nude
micewere divided into 2 groups: the group for assessment of tumor treatment efficacy (40 mice), the other
for assessment of immune cell rates in nude mouse spleen (9 mice). Nude mice were directely injected by
virus into thetumor with a dose of 107
PFU/mice, twice a week for 3 weeks, the control group wasinjected by
PBS. Immune cell rates in nude mice speen were assessed by flow cytometry analysis.
Results and conclusions: virus-treated groups hadthe tumor sizeand survival time that grewmore slowly
and was longer (p<0.05) those of the control group. The viral combination-treated group had the tumor size
and survival time that grew more slowly and was longer(p <0.05) than those of single virus-treated groups.
MeV and MuV were able to stimulate specific anti-tumor immune response in nude mice xenograft models.
Keywords: Nude mice, measles and mumps virus vaccine, colorectal cancer
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Copyright (c) 2019 Journal of Clinical Medicine Hue Central Hospital