Abstract

Air quality of operating room plays a very important role in SSI, microorganisms that present in the air will be a source of contamination of sterile instruments, clothing surgeon and invaded directly into the wound. Objective: Determine the number of microorganisms present in the operating room at the time before, during and after surgery, and learn some pollution causes of the operating room.
Subjects and Methods: 300 air samples taken in 15 operating rooms by AirPort MD8 machine of Sartorius company (Germany). The counting techniques, culture, identification of bacteria, fungi performed by technicians of Microbiology department.
Results: Before surgery, all of the hospital's operating rooms achived standards of microorganisms in the air (<180 cfu/m3). At the time of surgery, the total number of microorganisms began to rise in all operating rooms, ranged from 80.2 to 235.4 cfu/m3, with 20% of the operating rooms had microorganisms exceeded 180 cfu/m3. Ending of surgeries, surgical day quantity of bacteria, fungi in the operating rooms greatly increased compare with before surgery. When adding one person in the operating room, the risk of the number of microorganisms in the operating room air got over the threshold of 180 cfu/m3, increased by of 62%, number of the number of people > 6 that risk increased by 133%. The number of bacteria in the operating room was closely related to the opening and closing times (p <0.05).