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Abstract
Objective: Determinating the MSC counts after culturing cells from two different culture environments.
Research methods: A sample from human adipose tissue.
Results: After 20 days of culturing cells, MSC counts are 4.5x105 cells/ml on the environment 1 and 85.5% cell growth efficiency. MSC counts on the environment 2 are 2,2 x 105 cells/ml and 70,7% cell growth efficiency.
Conclusion: The cells cultured on the enviroment DMEM/F12, 10% FBS, 1% antibiotic/antimycotic have the highest mononuclear cell count: (4.5 ± 0.4) x 105 cells/ml and 85.5% cell growth efficiency.
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