Abstract
Objectives: The aims of this study was to use DNA sequencing to analyze the mutations of targeted genes (katG, rpoB) in INH-, and RIF-resistant M. tuberculosis isolated in Hue Central Hospital.
Methods: Drug susceptibility testing (DST) was determined by using standard conventional proportional method for 214 culture isolates. The sequencing was used for detecting mutations of eighty-one M. tuberculosis strains including 12 INH-sensitive strains and 34 INH-resistant strains, 12 RIF-sensitive strains and 23 RIF-resistant strains.
Results:
-The rate of drug resistant antituberculous was 24.3%. DST revealed that INH and STM had the highest resistant rate (18.2%), followed by RIF (11.2%), and the lowest resistant rate was that of EMB (7.9%).
- There were no mutations in the katG and rpoB genes in M. tuberculosis isolates sensitive to INH and RIF. The rate of mutation on katG gene of INH-resistant M. tuberculosis strains was 61.8%. Most mutations were found in katG gene codon 315 (90.5%). The rate of mutation on rpoB gene of RIF-resistant M. tuberculosis strains was 86.9%. We detected 15 mutation codon positions on rpoB gene, and the most prevalent mutations were in codons 531 (32.3%), 516 (16.1%), and 526 (12.9%). Rate of mutations located in 81-bp core region of rpoB gene was 67,7%. Most of Rifampicin resistant isolates are also resistant to Isoniazid, mutation in rpoB gene became important as a surrogate marker for MDR-TB.
Conclusions: Detection of mutations in the katG codon 315 and the hot-spot region of rpoB would be useful for rapid detection of INH and RIF resistance.
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| Published | 01-01-2015 | |
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| Issue | No. 27 (2015) | |
| Section | Original article | |
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